complete medium for keratinocyte culture kurabo industries  (Kurabo industries)

Journal: Theranostics

Article Title: Acceleration of chronic wound healing by bio-inorganic polyphosphate: In vitro studies and first clinical applications

doi: 10.7150/thno.67148

Figure Lengend Snippet: Wound healing. ( A ) The adult human skin organ consists of an epidermis and a dermis layer. While the epidermis is built of keratinocytes that are layered in four distinct strata, from basal cell layers to prickle and granular to horny ones. The dermis is composed of collagenous, reticular, and elastic fibers and primarily comprises fibroblasts but also immune cells (macrophages, astrocytes, and T-cells). The dermis is interspersed with blood and lymph capillaries. ( B ) When blood capillaries are wounded and damaged, platelets originating from megakaryocytes infiltrate into the wound area (also including the epidermis) and initiate blood clotting through a series of activation processes. The fibrin clot is formed, which seals the damaged tissue. During re-epithelialization and angiogenesis the extracellular matrix is reconstituted.

Article Snippet: The cells were cultivated in complete epidermal keratinocyte culture medium (#SCMK001, Sigma-Aldrich), as described , , and plated into 24-well plates coated with 200 µL of either polyP-free “Ma/Col” or polyP-containing “Ma/Col-Ca-polyP-NP”.

Techniques: Coagulation, Activation Assay

Journal: Theranostics

Article Title: Acceleration of chronic wound healing by bio-inorganic polyphosphate: In vitro studies and first clinical applications

doi: 10.7150/thno.67148

Figure Lengend Snippet: Keratinocytes that readily attach to collagen-based matrices. (A and B) Lower abundance of keratinocytes attached to the polyP-free matrix, “Ma/Col”, in contrast to cells (c) attached to ( C and D ) “Ma/Col-Ca-polyP-NP”. The matrices were overlayed with cells (4 • 10 4 cells/mL) for 24 h. After incubation, images were taken by ESEM. Noticeable are the microvilli (mv; circled) developed by keratinocytes on “Ma/Col-Ca-polyP-NP”.

Article Snippet: The cells were cultivated in complete epidermal keratinocyte culture medium (#SCMK001, Sigma-Aldrich), as described , , and plated into 24-well plates coated with 200 µL of either polyP-free “Ma/Col” or polyP-containing “Ma/Col-Ca-polyP-NP”.

Techniques: Incubation

Journal: Theranostics

Article Title: Acceleration of chronic wound healing by bio-inorganic polyphosphate: In vitro studies and first clinical applications

doi: 10.7150/thno.67148

Figure Lengend Snippet: Collagen-based mats and wetting solution. (A to D) Migration propensity of keratinocytes on collagen-based mats. The cells were seeded either on ( A and B ) “Ma/Col” or on ( C and D ) “Ma/Col-Ca-polyP-NP” and incubated in medium/serum for 24 h or 48 h; ESEM. Cells, seeded on “Ma/Col” remain on the surface of the matrix ( A ) and do not migrate into the mat ( B ). In contrast, after incubation on “Ma/Col-Ca-polyP-NP” the cells (c) invade ( C ) from a dense layer on the surface of the mats into their lower layers ( D ) after a 48 h incubation period. Five parallel samples were analyzed. (E to G) Moisturizing of the wound with the sterile wetting solution “WetSol”. Collagen mat before ( E ) and ( F ) after moistening with “WetSol”. The treated, moisturized sample was kept in a humid chamber for 6 h and then processed for microscopic analysis (SEM). During this period a coacervate (Coa) layer is formed on the surface of the mat. ( G ) 10 mL of sterile “WetSol” were filled into 10 mL syringes fitted with a Luer-Lock-adapter.

Article Snippet: The cells were cultivated in complete epidermal keratinocyte culture medium (#SCMK001, Sigma-Aldrich), as described , , and plated into 24-well plates coated with 200 µL of either polyP-free “Ma/Col” or polyP-containing “Ma/Col-Ca-polyP-NP”.

Techniques: Migration, Incubation